Journal: Endocrine journal

Article Title: Activation of Src-ATF1 pathway is involved in upregulation of Nox1, a catalytic subunit of NADPH oxidase, by aldosterone.

doi: 10.1507/endocrj.k10e-383

Figure Lengend Snippet: Fig. 1 PP2 but not AG1478 or GM6001 suppresses aldosterone-induced Nox1 expressions Nox1 mRNA expression in A7r5 cells. Results are shown as qPCR data. (A) Effect of PP2 on the Ald-induced Nox1 expression. Expression of Nox1 with indicated concentration of aldosterone and different concentrations of PP2 in VSMCs. (B) Effect of AG1478 on the Ald- induced Nox1 expression in VSMCs. Expression of Nox1 with indicated concentration of aldosterone and different concentrations of AG1478. (C) Effect of GM6001 on the Ald-induced Nox1 expression. Expression of Nox1 with indicated concentration of aldosterone and different concentrations of GM6001 in VSMCs. qPCR was performed as described in the Material and Methods section. Bars represent the means ±SEM of three experiments. *P < 0.05 vs. control group, #P < 0.05 vs. aldosterone group. Ald, aldosterone.

Article Snippet: The A7r5 rat aortic smooth muscle cell line was obtained from the American Type Culture Collection (Rockville, MD).

Techniques: Expressing, Concentration Assay, Control

Journal: Cellular & Molecular Biology Letters

Article Title: Heat shock protein 27 plays a protective role in thoracic aortic dissection by promoting cell proliferation and inhibiting apoptosis

doi: 10.1186/s11658-017-0056-y

Figure Lengend Snippet: Expressions of Hspb1 and HSP27 in A10 cells. a – Lentiviral infection is effective for overexpressing HSP27 in A10 cells, as shown by transfection with the vector containing the green fluorescent protein (GFP) gene, which makes the cells glow green under ultraviolet light. b – Quantitative PCR showing the Hspb1 expression level of non-infected A10 cells (non-infected control), A10 cells infected with Lenti6.3-EGFP empty vector (LVUT EGFP ), and A10 cells infected with LVUT Hspb1 (LVUT Hspb1 ). p < 0.05 between each pair of groups. Non-infected control, n = 4; LVUTE GFP , n = 4; LVUT Hspb1 , n = 4. c – A10 cells infected with LVUT Hspb1 express higher HSP27 protein levels than LVUT EGFP and non-infected A10 cells. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control protein

Article Snippet: A10 rat aortic SMCs (ATCC CRL 1476; American Type Culture Collection) were grown in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; GIBCO, Invitrogen Inc.) containing 10% fetal bovine serum (FBS; GIBCO, Invitrogen Inc.) in a humidified incubator with 5% CO 2 at 37 °C.

Techniques: Infection, Transfection, Plasmid Preparation, Real-time Polymerase Chain Reaction, Expressing, Control

Journal: Cellular & Molecular Biology Letters

Article Title: Heat shock protein 27 plays a protective role in thoracic aortic dissection by promoting cell proliferation and inhibiting apoptosis

doi: 10.1186/s11658-017-0056-y

Figure Lengend Snippet: A10 cells were treated with 2 mmol/l of H 2 O 2 for 2 h. Cell proliferation was assayed with an MTT assay kit. H 2 O 2 inhibited cell proliferation dramatically, but the inhibition rate was lower in A10 cells infected with LVUT Hspb1 than in A10 cells infected with LVUT EGFP ( p < 0.001). There were no significant differences between the non-infected control and LVUT EGFP ( n = 3)

Article Snippet: A10 rat aortic SMCs (ATCC CRL 1476; American Type Culture Collection) were grown in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; GIBCO, Invitrogen Inc.) containing 10% fetal bovine serum (FBS; GIBCO, Invitrogen Inc.) in a humidified incubator with 5% CO 2 at 37 °C.

Techniques: MTT Assay, Inhibition, Infection, Control

Journal: Cellular & Molecular Biology Letters

Article Title: Heat shock protein 27 plays a protective role in thoracic aortic dissection by promoting cell proliferation and inhibiting apoptosis

doi: 10.1186/s11658-017-0056-y

Figure Lengend Snippet: A10 cells were treated with 800 μmol/l of H 2 O 2 for 24 h. Cell apoptosis was assayed using an Annexin V-PE/7-AAD Apoptosis Detection Kit, and detected using FACS. In the dot plots, the lower left quadrant indicates a live cell population, the lower right quadrant indicates an early apoptotic cell population, and the upper right quadrant indicates a late apoptotic or dead cell population. H 2 O 2 -induced cell apoptosis was significantly attenuated in A10 cells infected with LVUT Hspb1 compared with A10 cells infected with LVUT EGFP (*p < 0.001). The rate of apoptosis showed no significant difference between the non-infected control and LVUT EGFP (n = 3)

Article Snippet: A10 rat aortic SMCs (ATCC CRL 1476; American Type Culture Collection) were grown in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; GIBCO, Invitrogen Inc.) containing 10% fetal bovine serum (FBS; GIBCO, Invitrogen Inc.) in a humidified incubator with 5% CO 2 at 37 °C.

Techniques: Infection, Control

Journal: Cellular & Molecular Biology Letters

Article Title: Heat shock protein 27 plays a protective role in thoracic aortic dissection by promoting cell proliferation and inhibiting apoptosis

doi: 10.1186/s11658-017-0056-y

Figure Lengend Snippet: A10 cells were treated with 2 mmol/l of H 2 O 2 for 2 h. Superoxide dismutase (SOD) activity and lipid peroxidation were tested to reflect the level of oxidative stress. Overexpression of HSP27 significantly increased SOD activity ( a ) and decreased the malondialdehyde level ( b ) in A10 cells infected with LVUT Hspb1 compared with A10 cells infected with LVUT EGFP . These two indexes showed no significant difference between non-infected controls and LVUT EGFP (n = 3)

Article Snippet: A10 rat aortic SMCs (ATCC CRL 1476; American Type Culture Collection) were grown in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; GIBCO, Invitrogen Inc.) containing 10% fetal bovine serum (FBS; GIBCO, Invitrogen Inc.) in a humidified incubator with 5% CO 2 at 37 °C.

Techniques: Activity Assay, Over Expression, Infection